| Group | Club/Team Name | Team ID | Head Coach |
| 447-6 | Ace Sport Academy ACE United 15UB |
0447-01RB10-0006 | Brandon Grandgenett |
| 426-17 | Adel DeSoto Soccer Club ADMSC B15A |
0426-01RB10-0017 | Zachary Plagman |
| 414-2 | Ames Soccer Club Ames SC 15u Boys - ISL CENTRAL |
0414-01RB09-0002 | Ollie Johnston |
| 401-3 | Ballard Soccer Club 15U Ballard Coed |
0401-01RB09-0003 | Ray Franks |
| 402-22 | Bondurant Soccer Club BSC U15 Blue |
0402-01RB10-0022 | Brandon Cook |
| 402-17 | Bondurant Soccer Club BSC U15 White |
0402-01RB10-0017 | Brandon Cook |
| 427-2 | Boone Soccer Club Boone 14/15U Coed |
0427-01RB10-0002 | Michelle Berei |
| 415-15 | Collins-Maxwell Rec Soccer club Collins-Maxwell U15 |
0415-01RB10-0015 | Jason Bell |
| 448-2 | Christian Soccer Academy CSA U15/16 |
0448-01RB09-0002 | Darlington Blayon |
| 417-1 | Earlham Soccer Club Earlham C16U |
0417-01RB09-0001 | Jackie Kosloske |
| 405-2 | Fort Dodge Soccer Association FDSA U15 Chaos |
0405-01RB10-0002 | Nicholas Huse |
| 405-3 | Fort Dodge Soccer Association FDSA U16 Chaos |
0405-01RB09-0003 | Mary Heldorfer |
| 430-27 | Indianola Soccer Tribe Indianola Soccer Tribe B15A |
0430-01RB10-0027 | Wladimir Rosales |
| 431-18 | Iowa Rush South Iowa Rush South Fall 2024 B16 |
0431-01RB09-0018 | Bailey Hamilton |
| 419-8 | Marshalltown Area Soccer Club Marshalltown Area Soccer Club - Eagles |
0419-01RB07-0008 | David Torres |
| 420-16 | North Polk United Soccer Club NPU B15A |
0420-01RB10-0016 | Crystal Lee |
| 425-11 | Spring Creek FC Spring Creek FC - B2009 |
0425-01RB09-0011 | Jason Van Arkel |
| 423-7 | WCV United Soccer Club WCV United U15/16 Co-Ed |
0423-01RB09-0007 | Mike Algreen |
| 437-8 | Winterset Soccer Club Winterset Socc - B2009 |
0437-01RB09-0008 | Caleigh Dowie |
| 444-1 | Woodward Granger Soccer Club Woodward Granger U14 Boys |
0444-01RB11-0001 | Colleen Salomon |