| Group | Club/Team Name | Team ID | Head Coach |
| 401-5 | Ballard Soccer Club 12U Ballard Girls |
0401-01RG13-0005 | Brett Fuller |
| 402-15 | Bondurant Soccer Club BSC G12 Blue |
0402-01RG13-0015 | Brock Brown |
| 428-8 | Carlisle Soccer Club CSC U12A Girls |
0428-01RG13-0008 | Heather Allen |
| 429-23 | Dallas Center Grimes Soccer Club DCG G11/12A |
0429-01RG13-0023 | Jeffrey Engh |
| 441-33 | Des Moines Soccer Club DMSC 12U Girls Rec |
0441-01RG13-0033 | Michael Thibodeau |
| 406-1 | Gilbert Soccer Club Gilbert Soccer Club - G2013 |
0406-01RG13-0001 | n/a |
| 430-39 | Indianola Soccer Tribe Indianola Soccer Tribe G12A |
0430-01RG13-0039 | Jeff Schortmann |
| 431-10 | Iowa Rush South Iowa Rush South Fall 2024 G12A |
0431-01RG13-0010 | Russell Goodman |
| 431-11 | Iowa Rush South Iowa Rush South Fall 2024 G12B |
0431-01RG13-0011 | Sarah Treft |
| 418-27 | J-Hawk Soccer Club J-Hawk SC 12UG |
0418-01RG13-0027 | Diane Baker |
| 432-3 | Knoxville Soccer Club Knoxville Socc - G2013B |
0432-01RG13-0002 | Andrew Schmidt |
| 432-2 | Knoxville Soccer Club Knoxville Socc - G2013A |
0432-01RG13-0003 | Jenna Weldon |
| 438-3 | Mahaska County Soccer Club MCSC 12u All Girls Lightning Bolts |
0438-01RG13-0003 | Seane San |
| 408-10 | Nevada Soccer Club N12R |
0408-01RG13-0010 | Ted Rasmusson |
| 409-13 | Newton Area Soccer Association NASA G12A |
0409-01RG13-0013 | Johannah Larson |
| 434-7 | Norwalk Soccer Club NSC 12U Girls |
0434-01RG13-0007 | James Joyce |
| 410-5 | Panorama Soccer Club Panorama Soccer Club - G2013 12/11U |
0410-01RG13-0005 | Jotham Arber |
| 421-44 | Pleasant Hill Soccer Club PHSC GU12A |
0421-01RG13-0044 | Michael Neal |
| 425-15 | Spring Creek FC Spring Creek FC - G2013 |
0425-01RG13-0015 | Jeff Kuney |
| 413-1 | Van Meter Youth Soccer Club Van Meter U11/12 A Girls |
0413-01RG13-0001 | Andrew Horsley |
| 413-2 | Van Meter Youth Soccer Club Van Meter U11/12 B Girls |
0413-01RG13-0002 | Brad Williams |
| 413-3 | Van Meter Youth Soccer Club Van Meter U11/12 C Girls |
0413-01RG13-0003 | n/a |
| 437-2 | Winterset Soccer Club Winterset Socc - G2013 |
0437-01RG13-0002 | Cody Sullivan |